FAT32 file size limit

Don't forget about the cli utility, convert

convert E: /FS:NTFS

You can perform this on a device that has files on it such as a USB disk but probably best to take a backup beforehand ;). Just make sure that you have sufficient free space on the partition, so that there’s room for temporary files that are created during the process; note that Windows will warn you if there is not enough space on the partition.